CD4 Antibody

SKU: orb749428

Description

CD4 is a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigens and is also a receptor for the human immunodeficiency virus. This protein is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, FACS
• Dilution Range: ELISA (order BSA/sodium azide-free format for coating),Flow cytometry: 1-2ug/million cells
• Reactivity: Human
• Application Notes:
  Optimal dilution of the CD4 antibody for a specific application should be determined by researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: C4/206
• Immunogen: Recombinant human CD4 protein was used as immunogen for the CD4 antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Flow cytometry staining of lymphocyte gated human PBM cells with CD4 antibody; Gray = unstained, Green = CD4 antibody.

SDS-PAGE Analysis of Purified, BSA-Free CD4 Antibody (clone C4/206). Confirmation of Integrity and Purity of the Antibody.

Quick Database Links

UniProt

P01730