CD4 antibody

• Catalog Number: orb4830
• Category: Antibodies
• Description: CD4 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC-P, WB
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CD4. Please contact us for the exact immunogen sequence. The peptide is available as orb374775.
• Concentration: - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml
• Dilution range: IHC-P: 1:500, IF/ICC: 1:200
• Form/Appearance: 10 mM PBS, 0.02% sodium azide
• Purity: Polyclonal antibodies are purified by peptide affinity chromatography
• Conjugation: Unconjugated
• MW: 51 kDa
• Target: CD4
• Entrez: 920
• UniProt ID: P01730, P05540, P06332
• NCBI: 000616, 000607, 41, 11
• Storage: Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
• Alternative names: Anti-CD 4 antibody, Anti-CD4 (L3T4) antibody, Anti
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Su, Jiyan et al. Protective Effect of Pogostone on 2,4,6-Trinitrobenzenesulfonic Acid-Induced Experimental Colitis via Inhibition of T Helper Cell Front Pharmacol, 8, 829 (2017)

PubMed: 29204117

Applications

IHC

Reactivity

Rat

Romero-Moreno, Ricardo et al. The CXCL5/CXCR2 axis is sufficient to promote breast cancer colonization during bone metastasis Nat Commun, 10, 4404 (2019)

PubMed: 31562303

Applications

IHC

Reactivity

Mouse

WB analysis of human breast cancer (lane 1), human thyroid cancer (lane 2), human endometrial cancer (lane 3), human ovarian cancer (lane 4) using CD4 antibody (1 ug/ml)

WB analysis of human breast tumor (lane 1), human mammary fibroma (lane 2), human breast cancer (lane 3) using CD4 antibody (1 ug/ml)

IHC-P image of human thyroid carcinoma tissue using CD4 antibody (2.5 ug/ml)

Western blot analysis of rat thymus (lane 1), rat liver (lane 2), mouse spleen (lane 3), mouse samll intestine (lane 4) using CD4 antibody (1 ug/ml)

Immunofluorescence image of mouse skin tissue using CD4 antibody (2.5 ug/ml)

Immunohistochemical staining of paraffin embedded mouse skin tissue using CD4 antibody (2.5 ug/ml)

IHC-P image of rat skin tissue using CD4 antibody (2.5 ug/ml)

Immunohistochemical staining of rat skin tissue using anti-CD4 (2.5 ug/ml)

IHC-P staining of rat colon tissue using CD4 antibody (5 ug/ml)

IHC-P staining of human thyroid carcinoma tissue using CD4 antibody (2.5 ug/ml)

Immunohistochemical staining of pig large intestines tissue using anti-CD4 (dilution of primary antibody - 1:200)

Immunohistochemical staining of paraffin embedded pig large intestines tissue using CD4 antibody (2.5 ug/ml)

IHC-P image of pig large intestines tissue using anti-CD4 (2.5 ug/ml)

Immunofluorescence analysis of mouse skin tissue using CD4 antibody (2.5 ug/ml)

Immunohistochemical staining of paraffin embedded mouse skin tissue using CD4 antibody (2.5 ug/ml)

Immunohistochemical staining of mouse lymph node tissue using anti-CD4 (5 ug/ml)

Western blot analysis of human breast tumor (lane 1), human mammary fibroma (lane 2), human breast cancer (lane 3) using CD4 antibody (1 ug/ml)