CD4 Antibody

• Catalog Number: orb640142
• Category: Antibodies
• Description: CD4 is a member of the Ig superfamily, primarily expressed on most thymocytes, a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a coreceptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosine kinase, lck. CD4 is expressed by the majority of thymocytes, most helper T cells, a subset of NK-T cells and weakly by dendritic cells and macrophages. CD4 plays an important role in the development of T cells and is required for mature T cells to function optimally.This antibody blocks helper T cell responses to MHC class II antigens, including cytolysis, proliferation, allogeneic B cell help, and release of lymphokines.
• Species/Host: Rat
• Clonality: Monoclonal
• Clone Number: GK1.5
• Tested applications: ELISA, FACS, IF, NeA
• Reactivity: Mouse
• Isotype: Rat IgG2b, kappa
• Immunogen: Mouse CTL clone V4 was used as the immunogen for the CD4 antibody.
• Dilution range: ELISA (order BSA-free format for coating & Azide),Neutralization Studies (order BSA/azide-free format),Flow cytometry: 1-2ug/million cells in 0.1ml,Immunofluorescence: 1-2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This CD4 antibody is available for research use only.
• UniProt ID: P06332
• Storage: Store the CD4 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.