CD4 Antibody

SKU: orb2637030

Description

Recognizes a protein of 55kDa, identified as CD4. It is a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigens and is also a receptor for the human immunodeficiency virus. This protein is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. The majority of peripheral T-cell lymphomas are derived from the T-helper/regulatory cell subset so that most mature T-cell neoplasms are CD4+/CD8-. Anti-CD4 is used in the immunohistochemical staining of lymphoproliferative disorders to evaluate tumors with CD4 aberrant expression.

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, FACS
• Dilution Range: ELISA (order BSA-free format for coating),Flow cytometry: 1-2ug/million cells
• Reactivity: Human
• Application Notes:
  Optimal dilution of the CD4 antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2b, kappa
• Clone No.: CD4/1604
• Immunogen: A portion of amino acids 245-392 was used as the immunogen for the CD4 antibody.
• Purification: Protein A/G affinity
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

FACS staining of lymphocyte-gated human PBM cells with CD4 antibody (red, clone CD4/1604) and isotype control (gray).

SDS-PAGE analysis of purified, BSA-free CD4 antibody (clone CD4/1604) as confirmation of integrity and purity.

Quick Database Links

UniProt

P01730