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Description
Research Area
Images & Validation
−| Tested Applications | IHC, IHC-P, WB |
|---|---|
| Dilution Range | IHC, IHC-P (10 µg/ml), WB (1:2000) |
| Reactivity | Human, Monkey |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Clone No. | 5D9 |
| Immunogen | Full length human recombinant protein of human CD4 (NP_000607) produced in HEK293T cell. |
| Target | CD4 |
| Purification | Protein A/G purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% rAlbumin |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Anti-CD4 antibody IHC staining of human spleen, lymphocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Western blot of extracts (35 ug) from 9 different cell lines by using anti-CD4 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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CD4 Antibody (orb1539454)
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