CD4 Antibody

SKU: orb1248089

Description

CD4 Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, FC, WB
• Reactivity: Human
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 30kDa band observed in Human Spleen lysates (calculated MW of 31.3kDa according to NP_001181943.1). Recommended concentration: 1-3µg/mL. Primary incubation was 1 hour.Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-KNKEVSVKRVTQDPK
• Target: CD4
• Molecular Weight: Approx 30kDa
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD4, CD4 molecule, CD4mut, CD4 antigen (p55), CD4 receptor, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

orb1248089 (1 ug/ml) staining of Human Spleen lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248089 Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line).

Quick Database Links

Gene Symbol

CD4

UniProt

P01730

RefSeq (Protein):NP_000607.1, NP_001181943.1