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CD4 antibody (MSVA-004R)
Catalog Number: orb2276554
| Catalog Number | orb2276554 |
|---|---|
| Category | Antibodies |
| Description | CD4 antibody validated for immunohistochemistry on 76 different Normal Tissues |
| Clonality | Monoclonal |
| Species/Host | Rabbit |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Immunogen | Recombinant fragment (around aa 216-396) of human CD4 protein (exact sequence is proprietary) |
| UniProt ID | P01730 |
| Tested applications | IHC |
| Dilution range | 1:100-1:200 |
| Application notes | Positive Control: Tonsil: A moderate to strong membranous CD4 immunostaining should be seen in a subset of T-lymphocytes which is predominantly located in the interfollicular area. Macrophages in the germinal centres should show a moderate CD4 positivity. Liver: Kupffer cells and endothelial cells in the liver sinusoids must show at least a moderate CD4 staining. Negative Control: Tonsil: B-cells and epithelial cells must not show any CD4 staining. Liver: Hepatocytes must not show any CD4 staining. Cellular Localization: Cell surface Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions. |
| Clone Number | Biorbyt-004R |
| Storage | Antibody with azide – store at 2 to 8°C. Antibody without azide – store at -20 to -80°C. Antibody is stable for 24 months. Non- hazardous. No MSD required. |
| Alternative names | L3T4; Leu3; Ly-4; Lymphocyte antigen CD4; p55; T c Read more... |
| Note | For research use only |
In tonsil tissue a strong CD4 immunostaining is seen in a fraction of T cells and in Langerhans cells of the tonsil surface epithelium. Epithelial cells are negative.
Liver - Endothelial CD4 positivity occurs at moderate intensity in liver sinusoids.
B CLL containing numerous CD4 positive lymphocytes.
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