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CD31 Antibody / PECAM-1

CD31 Antibody / PECAM-1

Catalog Number: orb749736

DispatchUsually dispatched within 5-10 working days
$ 764.00
Catalog Numberorb749736
CategoryAntibodies
DescriptionCD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 mAb reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence.
Species/HostMouse
ClonalityMonoclonal
Clone NumberC31.10
Tested applicationsFACS, IHC-P, WB
ReactivityHuman
IsotypeMouse IgG1, kappa
ImmunogenRecombinant human protein was used as the immunogen for the CD31 antibody.
Dilution rangeFlow cytometry: 1-2ug/million cells,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
PurityProtein G affinity chromatography
ConjugationUnconjugated
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Hazard InformationThis CD31 antibody is available for research use only.
UniProt IDP16284
StorageStore the CD31 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
NoteFor research use only
Application notesOptimal dilution of the CD31 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Expiration Date12 months from date of receipt.
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    FACS,  IHC-P,  WB

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    FACS,  IHC-P,  WB

    Human

    Rabbit

    Recombinant

    Unconjugated

    100 μg, 20 μg
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    IHC-P,  WB

    Human

    Mouse

    Recombinant

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    20 μg, 100 μg
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    IHC-P,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    100 μg, 20 μg
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