PECAM1 Antibody

SKU: orb1252132

Description

PECAM1 Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: FC, IF, IHC, WB
• Reactivity: Human
• Application Notes:
  Western blot: 1-2 µg/mLFlow Cytometry: 0.5-1ug/10e6 cellsIF: 1-2 µg/mLIHC (FFPE): 1-2 µg/mL for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)Titering of the CD31 antibody may be required for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1, k
• Clone No.: PCM25-1
• Immunogen: Recombinant human protein was used as the immunogen for this CD31 antibody.
• Target: PECAM1
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA', PECA1, CD31, PECAM1

FACS testing of Jurkat cells with an isotype control (green) and CD31 antibody (red).

IHC testing of colon carcinoma and CD31 antibody (PCM25-1).

Western blot testing of ThP1 cells using CD31 antibody (PCM25-1).

Quick Database Links

Gene Symbol

PECAM1

UniProt

P16284