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Catalog Number | orb1294236 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to CD3 (Intracellular domain) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit-IgG |
Immunogen | Synthetic peptide / Intracellular domain corresponding to Human/mouse CD3 |
Dilution range | Western Blot 1:1000 Immunofluorescence 1:300 – 1:400 Immunohistochemistry (Paraffin) 1:100 – 1:300 |
Conjugation | Unconjugated |
Target | CD3 Intracellular domain |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Anti-CD3 antibody at 1/1000 dilution. Lysates at 40 μg per lane. This blot was produced using a 15% SDS-PAGE. NC membrane was then blocked for an hour before being incubated with orb1294236 overnight at 4°C.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling CD3 with orb1294236 at 1/200.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling CD3 with orb1294236 at 1/200.
IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
98.00% | |
20-40 KDa (reducing condition) |
98.00% | |
38-55 KDa (reducing condition) |
98.00% | |
45-60 KDa (reducing condition) |