CD2BP2 Antibody

• Catalog Number: orb1247687
• Category: Antibodies
• Description: CD2BP2 Antibody
• Target: CD2BP2
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: PKRKVTFQGVGDE-C
• UniProt ID: O95400
• Tested applications: ELISA, IHC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 50kDa band observed in Human Lymph Node, Thymus and Tonsil lysates (calculated MW of 37.6kDa according to NP_006101.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Nishizawa et al, Proc Natl Acad Sci U S A. 1998 Dec 8;95(25):14897-902). Recommended concentration: 0.3-1.0ug/ml.Immunohistochemistry:In paraffin embedded Human Spleen shows nucleolar staining in select splenocytes. Recommended concentration: 5-10ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CD2BP2, FWP010, LIN1, Snu40, CD2 antigen (cytoplas
• Research Area: Cancer Biology
• Note: For research use only
• NCBI: NP_006101.1

orb1247687 (0.3 ug/ml) staining of Human Thymus lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1247687 (5 ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.