CD28 Antibody

• Catalog Number: orb1240012
• Category: Antibodies
• Description: CD28 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC, WB
• Predicted Reactivity: Bovine, Feline, Mouse, Rabbit, Rat
• Reactivity: Human
• Isotype: IgG
• Immunogen: CD28 antibody was raised against a peptide corresponding to 18 amino acids near the carboxy terminus of human CD28.The immunogen is located within the last 50 amino acids of CD28.
• Concentration: 1 mg/mL
• Dilution range: CD28 antibody can be used for Western blot at 1μg/mL. Antibody can also be used for Immunohistochemistry at 2 μg/mL. For Immunoflorescence start at 20 μg/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 28 kDa Observed: 26 kDa
• Target: CD28
• UniProt ID: P10747
• NCBI: P10747
• Storage: CD28 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: CD28 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: CD28 Antibody: CD28 Molecule, CD28 Antigen, Tp44,
• Note: For research use only
• Application notes: CD28 antibody can be used for Western blot at 1μg/mL. Antibody can also be used for Immunohistochemistry at 2 μg/mL. For Immunoflorescence start at 20 μg/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of CD28 in human spleen tissue lysate with CD28 antibody at 1 µg/mL in (A) the absence and (B) the presence of blocking peptide.

Immunohistochemistry of CD28 in human spleen tissue with CD28 antibody at 2 µg/ml.

Immunofluorescence of CD28 in human spleen tissue with CD28 antibody at 20 µg/ml.