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Catalog Number | orb749881 |
---|---|
Category | Antibodies |
Description | Recognizes a protein of a disulfide-linked 120kDa dimer, identified as CD27 (Workshop VI; Code 6T-028). CD27 is expressed on the majority of peripheral T cells, medullary thymocytes, memory-type B cells, and natural killer cells. It is a transmembrane phosphoglycoprotein that belongs to the tumor necrosis factor receptor (TNFR) superfamily. CD27 binds to its ligand CD70, a member of the TNF family, and induces T-cell co-stimulation and B-cell activation. It also interacts with TRAFs and is involved in activation of NFB and SAPK/JNK and induces apoptosis. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 203.6 |
Tested applications | FACS, IF |
Reactivity | Human |
Isotype | Mouse IgG3, kappa |
Immunogen | Sezary cells were used as the immunogen for the CD27 antibody. |
Dilution range | Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml |
Purity | Protein G affinity chromatography |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide |
Hazard Information | This CD27 antibody is available for research use only. |
UniProt ID | P26842 |
Storage | Store the CD27 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide). |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide |
Note | For research use only |
Application notes | Optimal dilution of the CD27 antibody should be determined by the researcher. |
Expiration Date | 12 months from date of receipt. |
ELISA, FA, FACS, Kinetics | |
Human | |
Monoclonal | |
Unconjugated |
IF, IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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