CD27 Antibody

• Catalog Number: orb757382
• Category: Antibodies
• Description: Anti-CD27 antibody(DM59), Rabbit mAb
• Target: CD27
• Clonality: Monoclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Purification: Purified from cell culture supernatant by affinity chromatography
• UniProt ID: P26842
• Tested applications: ELISA, FC, IHC, WB
• Dilution range: ELISA 1/5000-10000|Flow Cyt 1/100
• Application notes: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Antibody Type: Recombinant Antibody
• Clone Number: DM59
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CD27, TNFRSF7, S152, T14, Tp55
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Expi 293 cell line transfected with irrelevant protein (A) and human CD27 (B) were surface stained with Rabbit anti-CD27 monoclonal antibody 1 μg/ml (clone: DM59) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.

Flow cytometry data of serially titrated Rabbit anti-CD27 monoclonal antibody (clone: DM59) on Raji cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.

Affinity ranking of different Rabbit anti-CD27 mAb clones by titration of different concentration onto Raji cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.

Flow cytometry analysis of antigen binding of rabbit anti-human CD27 mAb (orb757382). (A) orb757382 does not bind to 293T cells that do not express CD27. (B) A clear peak shift of orb757382 was seen compared to the control when incubated with CD27-expressing Raji cells, indicating strong binding of orb757382 to CD27. Antibodies were incubated at 2 μg/mL.

Anti-CD27 antibody (SKU# orb757382) at 1/1000 dilution.Lane: Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate.Secondary: Goat Anti-Rabbit IgG H&L (HRP) at 1/5000 dilution.Predicted band size: 29 kDa.Observed band size: 55 kDa

A. orb757382 at 10 μg/ml staining CD27 in Raji cells by IHC (SKU# orb757382); B. orb757382 at 10 μg/ml staining CD27 in human tonsil tissue by IHC (SKU# orb757382).