CD27 antibody

SKU: orb2276509

Description

CD27 antibody validated for immunohistochemistry on 76 different Normal Tissues

Research Area

Immunology & Inflammation; Cell Biology

Images & Validation

• Tested Applications: IHC
• Dilution Range: 1:100-1:200
• Reactivity: Human
• Application Notes:
  Positive Control: Tonsil: A strong, membranous staining should be seen in the majority of interfollicular lymphocytes but also in a fraction of lymphocytes in the germinal centres and the mantle zone. Negative Control: Tonsil: CD27 staining should be absent in all epithelial cells and a fraction of lymphocytes. Cellular Localization: Cell Surface Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit according to the manufacturer’s directions.

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Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b, kappa
• Clone No.: MSVA-027M
• Immunogen: Recombinant human CD27 protein fragment (aa 28-170) (exact sequence is proprietary)
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

LPFS2; S152; T cell activation antigen S152; T-cell activation antigen CD27; T14; TNFRSF7; TNFSF7; Tp55; Tumor necrosis factor receptor superfamily member 7

Quick Database Links

UniProt

P26842