CD23 (Fc Epsilon RII) Antibody

• Catalog Number: orb1410377
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD23 (Fc Epsilon RII)
• Target: FCER2
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG2b
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Immunogen: Recombinant fragment (around aa 48-321) of human FCER2/CD23 protein (exact sequence is proprietary)
• UniProt ID: P06734
• MW: 37kDa (soluble form); 45kDa (membrane-bound form)
• Tested applications: IHC
• Clone Number: FCER2/6887
• Expression System: HEK293 cells
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: BLAST-2; C-type lectin domain family 4, member J;
• Research Area: Immunology & Inflammation, Signal Transduction
• Note: For research use only

Analysis of Protein Array containing more than 19000 full-length human proteins using CD23-Monospecific Mouse Monoclonal Antibody (FCER2/6887). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

IHC analysis of formalin-fixed, paraffin-embedded human lymph node. Membrane staining using FCER2/6887 at 2 ug/ml in PBS for 30 min RT. HIER: Tris/EDTA, pH9.0, 45 min. 2°C: HRP-polymer, 30 min. DAB, 5 min.

IHC analysis of formalin-fixed, paraffin-embedded human tonsil. Membrane staining using FCER2/6887 at 2 ug/ml in PBS for 30 min RT. Inset: PBS instead of primary antibody; secondary only negative control.

SDS-PAGE Analysis of Purified CD23-Monospecific Mouse Monoclonal Antibody (FCER2/6887). Confirmation of Purity and Integrity of Antibody.