CD22 Antibody

SKU: orb606498

Description

Recognizes a protein of 130-140kDa, identified as CD22 (also known as BL-CAM). CD22 expression is restricted to normal and neoplastic B cells and is absent from other haemopoietic cell types. In B-cell ontogeny, CD22 is first expressed in the cytoplasm of pro-B and pre-B cells, and on the surface as B cells mature to become IgD+. It is not expressed by plasma cells, CD22 is found highly expressed in follicular mantle and marginal zone B-cells, and while germinal center B-cells are relatively weak. CD22 is a member of the immunoglobulin superfamily and serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. It also associates with tyrosine kinases and play a role in signal transduction and B-cell activation.

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, FACS, IF, IHC-P, WB
• Dilution Range: ELISA: 2-4ug/ml (order BSA/azide-free format),Flow cytometry: 1-2ug/10^6 cells,Western blot: 1-2ug/ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  Optimal dilution of the CD22 antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: BLCAM/1795
• Immunogen: Amino acids 52-178 from the human protein were used as the immunogen for the CD22 antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC testing of FFPE human tonsil tissue with CD22 antibody (clone BLCAM/1795). HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human spleen tissue with CD22 antibody (clone BLCAM/1795). HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.

Western blot testing of FFPE human 1) Raji and 2) Ramos cell lysate with CD22 antibody (clone BLCAM/1795). Expected molecular weight: 76/95 kDa (alpha/beta, unmodified), 130-150 kDa (glycosylated).

Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using CD22 antibody (clone BLCAM/1795). These results demonstrate the foremost specificity of the BLCAM/1795 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free CD22 antibody (clone BLCAM/1795) as confirmation of integrity and purity.

Quick Database Links

UniProt

P20273