CD22 Antibody

• Catalog Number: orb153503
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD22
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 2H1C4
• Tested applications: FC, ICC, IHC, WB
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: Purified recombinant fragment of human CD22 (AA: 621-725) expressed in E. Coli.
• Antibody Type: Primary Antibody
• Concentration: Approximately 1mg/ml (varies from batch to batch). Please inquire for precise concentration.
• Dilution range: WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; ICC: 1/50 - 1/200; FCM: 1/200 - 1/400; ELISA: 1/10000
• Conjugation: Unconjugated
• MW: 95.3kDa
• Target: CD22
• Entrez: 933
• UniProt ID: P20273
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Purified antibody in PBS with 0.05% sodium azide
• Alternative names: SIGLEC2; SIGLEC-2
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of HEK293 (Lane 1) HEK293 (Lane 2) cell lysate using CD22 antibody

Western blot analysis of L1210 (Lane 1), Hela (Lane 2), HEK293 (Lane 3), Jurkat (Lane 4), OCM-1 (Lane 5), A432 (Lane 6) NIH/3T3 (Lane 7) cell lysate using CD22 antibody

Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of CD22 antibody

Flow cytometric analysis of Hela cells lysates using CD22 antibody

Immunohistochemical analysis of formalin-fixed and paraffin-embedded cervical cancer tissues using CD22 antibody

Immunohistochemical analysis of formalin-fixed and paraffin-embedded cerebellum tissues using CD22 antibody

Western blot analysis of human CD22 using CD22 antibody