CD22 Antibody

SKU: orb534085

Description

Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules. [Uniprot]

Research Area

Epigenetics

Images & Validation

• Tested Applications: FACS, IF, WB
• Dilution Range: Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/10^6 cells,Immunofluorescence: 1-2ug/ml
• Reactivity: Human
• Application Notes:
  The stated application concentrations are suggested starting points. Titration of the CD22 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2b, kappa
• Clone No.: CDLA22-1
• Immunogen: Amino acids 52-178 from the human protein were used as the immunogen for the CD22 antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Western blot testing of human Raji cell lysate with CD22 antibody (clone CDLA22-1). Expected molecular weight: 76-150 kDa depending on glycosylation level.

Flow cytometry testing of human Ramos cells with CD22 antibody (clone CDLA22-1); Red = isotype control, Blue = CD22 antibody.

Immunofluorescent staining of human Ramos cells with CD22 antibody (clone CDLA22-1, green) and Reddot nuclear stain (red).

Quick Database Links

UniProt

P20273