CD20 Antibody

• Catalog Number: orb607192
• Category: Antibodies
• Description: Recognizes a protein of 30-33kDa, which is identified as CD20. It is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre-B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. This MAb can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood and B cell localization in tissues. It reacts with the majority of B-cells present in peripheral blood and lymphoid tissues and their derived lymphomas. In lymphoid tissue, germinal center blasts and B-immunoblasts are particularly reactive. It is a reliable antibody for ascribing a B-cell phenotype in known lymphoid tissues. Rarely, CD20-positive T-cell lymphomas have been reported. Reactivity has also been noted with Reed-Sternberg cells in cases of Hodgkin's disease, particularly of lymphocyte predominant type.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG2b, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Purity: Protein G affinity chromatography
• Immunogen: Amino acids 213-297 from the human protein were used as the immunogen for this CD20 antibody.
• UniProt ID: P11836
• Tested applications: ELISA, FACS, IF, IHC-P, WB
• Dilution range: ELISA: 2-4ug/ml (order BSA/azide-free format),Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: MS4A1/3409
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: CD20 antibody with azide can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
• Hazard Information: This CD20 antibody is available for research use only.
• Note: For research use only

IHC staining of FFPE human tonsil tissue with CD20 antibody (clone MS4A1/3409). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

Immunofluorescence staining of human Raji cells with CD20 antibody (green, clone MS4A1/3409) and Reddot nuclear stain (red).

Flow cytometry testing of human Raji cells with CD20 antibody (clone MS4A1/3409); Red=isotype control, Blue= CD20 antibody.

Western blot testing of human Raji lysate with CD20 antibody (clone MS4A1/3409). Predicted molecular weight ~33 kDa.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using CD20 antibody (clone MS4A1/3409). These results demonstrate the foremost specificity of the MS4A1/3409 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free CD20 antibody (clone MS4A1/3409) as confirmation of integrity and purity.