CD20 Antibody

• Catalog Number: orb607185
• Category: Antibodies
• Description: Recognizes a protein of 30-33kDa, which is identified as CD20. It is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre-B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. This MAb can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood and B cell localization in tissues. It reacts with the majority of B-cells present in peripheral blood and lymphoid tissues and their derived lymphomas. In lymphoid tissue, germinal center blasts and B-immunoblasts are particularly reactive. It is a reliable antibody for ascribing a B-cell phenotype in known lymphoid tissues. Rarely, CD20-positive T-cell lymphomas have been reported. Reactivity has also been noted with Reed-Sternberg cells in cases of Hodgkin's disease, particularly of lymphocyte predominant type.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG2b, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Amino acids 213-297 from the human protein were used as the immunogen for this CD20 antibody.
• UniProt ID: P11836
• Tested applications: ELISA, FACS, IF, IHC-P, WB
• Dilution range: ELISA: 2-4ug/ml (order BSA/azide-free format),Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: MS4A1/3411
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Research Area: Epigenetics
• Note: For research use only

Immunofluorescence staining of human Raji cells with CD20 antibody (green, clone MS4A1/3411) and Reddot nuclear stain (red).

IHC staining of FFPE human tonsil tissue with CD20 antibody (clone MS4A1/3411). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

Flow cytometry testing of human Raji cells with CD20 antibody (clone MS4A1/3411); Red=isotype control, Blue= CD20 antibody.

Western blot testing of human Raji lysate with CD20 antibody (clone MS4A1/3411). Predicted molecular weight ~33 kDa.

SDS-PAGE analysis of purified, BSA-free CD20 antibody (clone MS4A1/3411) as confirmation of integrity and purity.