SLAMF9 Rabbit Polyclonal Antibody

• Catalog Number: orb13313
• Category: Antibodies
• Description: SLAMF9 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Predicted Reactivity: Mouse
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human SLAMF9 (51-150/289aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, Flow-Cyt=1μg/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 30 kDa
• Target: SLAMF9
• UniProt ID: Q96A28
• RRID: AB_10747247
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: SLAM family member 9; CD2 family member 10; CD2F-1
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control (black line): Raji (black) (The cells were fixed with 2% paraformaldehyde (10 min), then permeabilized with PBST for 30 min on room temperature), Primary Antibody (rad line): Rabbit Anti-SLAMF9 antibody (orb13313), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (green line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, Dilution: 1 µg/Test.

Blank control: (mo)splenocytes (blue), Isotype Control Antibody: Rabbit IgG-FITC (orange), Primary Antibody Dilution: 1 µl in 100 µl 1X PBS containing 0.5% BSA (green).

Sample: A549 (human) cell Lysate at 40 ug, BV2 (mouse) cell Lysate at 40 ug, Primary: Anti-SLAMF9 (orb13313) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 30kD, Observed band size: 35 kD.