Cart summary

You have no items in your shopping cart.

CD19 Rabbit Polyclonal Antibody

Catalog Number: orb317567

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb317567
CategoryAntibodies
DescriptionCD19 Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsFC, IF, IHC-Fr, IHC-P
Predicted ReactivityBovine, Equine, Guinea pig, Porcine
ReactivityHuman, Mouse, Rat
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human CD19 (485-556/556aa)
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeIHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test
Form/AppearanceLiquid
ConjugationUnconjugated
MW95 kDa
TargetCD19
UniProt IDP15391
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesCD19_HUMAN; B-lymphocyte antigen CD19; B-lymphocyt
Read more...
NoteFor research use only
Expiration Date12 months from date of receipt.
CD19 Rabbit Polyclonal Antibody

Blank control (blue line): HL60 cells (blue). Primary Antibody (green line): Rabbit Anti-CD19 antibody (orb317567), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, dilution: 1 µg/Test. Protocol, The cells were fixed with 70% methanol (Overnight at 4°C). Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

CD19 Rabbit Polyclonal Antibody

Blank control: Raji (blue). Primary Antibody: Rabbit Anti-CD19 antibody (orb317567), dilution: 5 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA. Protocol, Primary antibody (orb317567, 5 µg/1x10^6 cells) were incubated for 30 min on the ice, followed by 1X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20000 events was performed.

CD19 Rabbit Polyclonal Antibody

Tissue/Cell: mouse fetal skin, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-CD19 Polyclonal Antibody, Unconjugated (orb317567) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

  • CD19 Polyclonal Antibody [orb1411191]

    IHC-P,  WB

    Human

    Rabbit

    Polyclonal

    Unconjugated

    100 μl
  • CD19 Antibody (N-term) [orb1935338]

    FC,  IF,  IHC-P,  WB

    Human

    Rabbit

    Polyclonal

    Unconjugated

    100 μl, 50 μl
  • CD19 purified MaxPab rabbit polyclonal antibody (D01P) [orb2294906]

    PLA,  WB

    Human, Mouse

    Rabbit

    Polyclonal

    Unconjugated

    100 μg
  • CD19 [orb1294296]

    FC,  IF,  IHC,  WB

    Human

    Rabbit

    Polyclonal

    Unconjugated

    25 μl, 100 μl
  • CD19 Rabbit Polyclonal Antibody [orb1699]

    FC

    Guinea pig, Porcine, Rat

    Human, Mouse

    Rabbit

    Polyclonal

    Unconjugated

    50 μl, 100 μl, 200 μl