CD19 Antibody

SKU: orb749432

Description

CD19 is a transmembrane glycoprotein that contains two extracellular immunoglobulin-like domains. It is present in both benign and malignant B-cells and is considered to be the most reliable surface marker of this lineage over a wide range of maturational stages.

Images & Validation

• Tested Applications: FACS, IF
• Dilution range: Flow cytometry: 1-2ug/10^6 cells,Immunofluorescence: 1-2ug/ml
• Reactivity: Human
• Application Notes:
  The stated application concentrations are suggested starting amounts. Variations in protocols, secondaries and substrates may require the anti-CD19 antibody to be titered up or down for optimal performance.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: CVID3/155
• Immunogen: Human CD19 recombinant protein was used as immunogen for this anti-CD19 antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

Flow cytometry testing of lymphocyte-gated human PBM cells with anti-CD19 antibody (clone CVID3/155); Gray = unstained cells, Orange = anti-CD19 antibody stained cells.

Flow cytometry testing of human Raji cells with anti-CD19 antibody (clone CVID3/155); Red = isotype control, Blue = anti-CD19 antibody.

Immunofluorescent staining of human Raji cells with anti-CD19 antibody (clone CVID3/155, green) and Reddot nuclear stain (red).

SDS-PAGE Analysis of Purified, BSA-Free CD19 Antibody (clone CVID3/155). Confirmation of Integrity and Purity of the Antibody.

Quick Database Links

UniProt

P15391