CD163 antibody

• Catalog Number: orb1333718
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD163
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: OTI2G12
• Tested applications: IF, IHC, WB
• Reactivity: Human
• Isotype: IgG1
• Immunogen: Full length human recombinant protein of human CD163(NP_004235) produced in HEK293T cell.
• Dilution range: WB 1:1000, IHC 1:150, IF 1:100
• Conjugation: Unconjugated
• MW: 125.3 kDa
• Target: CD163
• UniProt ID: Q86VB7
• NCBI: NM_004244
• Note: For research use only
• Application notes: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
• Expiration Date: 12 months from date of receipt.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD163 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD163.

Western blot analysis of extracts (35ug) from 3 tissue lysates by using anti-CD163 monoclonal antibody (1:250).

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CD163 mouse monoclonal antibody.

Anti-CD163 mouse monoclonal antibody (TA506380) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD163 (RC208557).