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Product Overview
Product Name CD163 antibody
Catalog Number orb13303
Species/HostRabbit
ReactivityGuinea pig, Human, Mouse, Rat
ConjugationUnconjugated
Custom conjugations
Tested applicationsICC, IF, IHC-P, WB
Immunogen

KLH conjugated synthetic peptide derived from human CD163. Please contact us for the exact immunogen sequence. The peptide is available as orb374759.

Target CD163
Alternative Names
Product Properties
Form/Appearance 10 mM PBS, 0.02% sodium azide
Concentration

- 100 μg (in 200 μl): 0.5 mg/ml
- 200 μg (in 400 μl): 0.5 mg/ml

Storage Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
Note For research use only.
Isotype IgG
ClonalityPolyclonal
Purity

Polyclonal antibodies are purified by peptide affinity chromatography

MW 125 kDa
Uniprot ID

Q86VB7 (Human); Q2VLH6 (Mouse); D3Z9U2 (Rat)

NCBI NP_004235.41; NM_004244.51
Entrez 9332
Product Description

Rabbit polyclonal antibody to CD163. This protein (also known as M130), belongs to a macrophage-associated antigen, which is a member of the scavenger receptor cysteine-rich (SRCR) superfamily. CD163 acts as a receptor that scavenges hemoglobin by mediating endocytosis of haptoglobin-hemoglobin complexes.

Application Notes
Dilution Range IHC-P: 1: 100-600, WB: 1:500, ICC: 1:100
Validation Images
Western blot analysis of rat lung tissue using anti-CD163 (dilution of primary antibody at 1:500)
Western blot analysis of rat lung tissue using anti-CD163 (dilution of primary antibody at 1:500)
Immunohistochemical staining of paraffin embedded human lung cancer tissue using CD163 antibody (2.5 ug/ml)
Immunohistochemical staining of paraffin embedded human lung cancer tissue using CD163 antibody (2.5 ug/ml)
Immunohistochemical staining of guinea pig colon tissue using anti-CD163 (2.5 ug/ml)
Immunohistochemical staining of guinea pig colon tissue using anti-CD163 (2.5 ug/ml)
Immunohistochemical staining of guinea pig colon tissue using anti-CD163 (2.5 ug/ml)
Immunohistochemical staining of guinea pig colon tissue using anti-CD163 (2.5 ug/ml)
IHC-P image of mouse stomach tissue using anti-CD163 (2.5 ug/ml)
IHC-P image of mouse stomach tissue using anti-CD163 (2.5 ug/ml)
Immunohistochemical staining of mouse stomach tissue using anti-CD163 (2.5 ug/ml)
Immunohistochemical staining of mouse stomach tissue using anti-CD163 (2.5 ug/ml)
Immunohistochemical staining of rat lymph tissue using CD163 antibody (2.5 ug/ml)
Immunohistochemical staining of rat lymph tissue using CD163 antibody (2.5 ug/ml)
IHC-P image of rat lymph tissue using anti-CD163 (2.5 ug/ml)
IHC-P image of rat lymph tissue using anti-CD163 (2.5 ug/ml)
Western blot analysis of guniea pig lymph (lane 1), rat brain (lane 2), mouse stomach (lane3), rat rectum (lane4), human gallbladder (lane 5), human lung cancer (lane 6) tissue using anti-CD163 (0.5 ug/ml)
Western blot analysis of guniea pig lymph (lane 1), rat brain (lane 2), mouse stomach (lane3), rat rectum (lane4), human gallbladder (lane 5), human lung cancer (lane 6) tissue using anti-CD163 (0.5 ug/ml)
Immunohistochemical staining of mouse skin tissue using CD163 antibody (dilution of primary antibody - 1:200)
Immunohistochemical staining of mouse skin tissue using CD163 antibody (dilution of primary antibody - 1:200)
Immunofluorescent analysis of mouse lung tissue using CD163 antibody (Primary antibody diluted to 1:100)
Immunofluorescent analysis of mouse lung tissue using CD163 antibody (Primary antibody diluted to 1:100)
Immunohistochemical staining of mouse liver tissue using anti-CD163 (dilution of primary antibody - 1:200)
Immunohistochemical staining of mouse liver tissue using anti-CD163 (dilution of primary antibody - 1:200)
Immunofluorescent image of mouse liver tissue using CD163 antibody (Primary antibody at 1:100)
Immunofluorescent image of mouse liver tissue using CD163 antibody (Primary antibody at 1:100)
IF analysis of mouse skin tissue using CD163 antibody (Dilution of primary antibody 1:100)
IF analysis of mouse skin tissue using CD163 antibody (Dilution of primary antibody 1:100)
Immunohistochemical staining of paraffin embedded mouse liver tissue using CD163 antibody. (Dilution of primary antibody 1:200)
Immunohistochemical staining of paraffin embedded mouse liver tissue using CD163 antibody. (Dilution of primary antibody 1:200)
Immunohistochemical staining of human colon cancer tissue using CD163 antibody. (Dilution of primary antibody 1:200)
Immunohistochemical staining of human colon cancer tissue using CD163 antibody. (Dilution of primary antibody 1:200)
Immunohistochemical staining of paraffin embedded human colon cancer tissue (orb13303 at 1:200) using CD163 antibody. (Dilution of primary antibody 1:200)
Immunohistochemical staining of paraffin embedded human colon cancer tissue (orb13303 at 1:200) using CD163 antibody. (Dilution of primary antibody 1:200)
Western blot analysis of human cell lysates using CD163 antibody (please check application notes for details)
Western blot analysis of human cell lysates using CD163 antibody (please check application notes for details)
IHC-P of human lung adenocarcinoma tissue using CD163 antibody (Dilution of primary antibody 1:200)
IHC-P of human lung adenocarcinoma tissue using CD163 antibody (Dilution of primary antibody 1:200)
IHC-P image of human thyroid carcinoma tissue using CD163 antibody (dilution of primary antibody at 1:500)
IHC-P image of human thyroid carcinoma tissue using CD163 antibody (dilution of primary antibody at 1:500)
WB analysis of human breast cancel 25 (Lane1), breast cancel 24 (Lane2) using CD163 antibody (dilution at 1:100)
WB analysis of human breast cancel 25 (Lane1), breast cancel 24 (Lane2) using CD163 antibody (dilution at 1:100)
Immunohistochemical staining of pig large intestines tissue using anti-CD163 (dilution of primary antibody - 1:200)
Immunohistochemical staining of pig large intestines tissue using anti-CD163 (dilution of primary antibody - 1:200)
IHC-P staining of pig lung tissue using anti-CD163 (dilution at 1:200)
IHC-P staining of pig lung tissue using anti-CD163 (dilution at 1:200)
Immunofluorescence image of rat skin tissue using anti-CD163 (dilution at 1:100)
Immunofluorescence image of rat skin tissue using anti-CD163 (dilution at 1:100)
WB analysis of human breast cancer (Lane 1), breast cancer (Lane 2), breast cancer (Lane 3) using anti-CD163 (dilution at 1:500)
WB analysis of human breast cancer (Lane 1), breast cancer (Lane 2), breast cancer (Lane 3) using anti-CD163 (dilution at 1:500)
Immunohistochemical staining of paraffin embedded mouse skin tissue using CD163 antibody (primary antibody at 1:200)
Immunohistochemical staining of paraffin embedded mouse skin tissue using CD163 antibody (primary antibody at 1:200)
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