CD163 antibody

• Catalog Number: orb13303
• Category: Antibodies
• Description: CD163 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IF, IHC-P, WB
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CD163. Please contact us for the exact immunogen sequence. The peptide is available as orb374759.
• Concentration: - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml
• Dilution range: IHC-P: 1: 100-600, WB: 1:500, ICC: 1:100
• Form/Appearance: 10 mM PBS, 0.02% sodium azide
• Purity: Polyclonal antibodies are purified by peptide affinity chromatography
• Conjugation: Unconjugated
• MW: 125 kDa
• Target: CD163
• Entrez: 9332
• UniProt ID: Q86VB7, D3Z9U2, Q2VLH6
• NCBI: 004235, 004244, 41, 51
• RRID: AB_10749283
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti-Cluster Of Differentiation antibody, anti-CD
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

SP Dai, CC Yang, Y Chin, WH Sun TDAG8-mediated distinct signaling pathways modulate the early and late phases of neuropathic pain iScience, (2024)

PubMed:

Applications

IHC

Reactivity

Mouse

Mengxuan Li,Nan Che,Xingzhe Liu,Yanhua Xuan,Yu Jin Dauricine regulates prostate cancer progression by inhibiting PI3K/AKT-dependent M2 polarization of macrophages Biochemical Pharmacology, 217, 115838 (2023)

PubMed: 37778445

Chen-Roetling, Jing et al. Haptoglobin increases the vulnerability of CD163-expressing neurons to hemoglobin J Neurochem, 139, 586-595 (2016)

PubMed: 27364920

Mertens, Barbara et al. Investigation of tumor-tumor interactions in a double human cervical carcinoma xenograft model in nude mice Oncotarget, 9, 21978-22000 (2018)

PubMed: 29774117

de Vries, M. R. et al. Blockade of vascular endothelial growth factor receptor 2 inhibits intraplaque haemorrhage by normalization of plaque neovessels J Intern Med, 285, 59-74 (2019)

PubMed: 30102798

Heindryckx, Femke et al. Inhibition of the placental growth factor decreases burden of cholangiocarcinoma and hepatocellular carcinoma in a transgenic mouse model Eur J Gastroenterol Hepatol, 24, 1020-1032 (2012)

PubMed: 22772092

Koronyo, Yosef et al. Therapeutic effects of glatiramer acetate and grafted CD115⁺ monocytes in a mouse model of Alzheimer's disease Brain, 138, 2399-2422 (2015)

PubMed: 26049087

Gibson, D. L. et al. Maternal exposure to fish oil primes offspring to harbor intestinal pathobionts associated with altered immune cell balance Gut Microbes, 6, 24-32 (2015)

PubMed: 25559197

Koronyo-Hamaoui, Maya et al. Peripherally derived angiotensin converting enzyme-enhanced macrophages alleviate Alzheimer-related disease Brain, 143, 336-358 (2020)

PubMed: 31794021

Immunohistochemical staining of paraffin embedded mouse skin tissue using CD163 antibody (primary antibody at 1:200)

WB analysis of human breast cancer (Lane 1), breast cancer (Lane 2), breast cancer (Lane 3) using anti-CD163 (dilution at 1:500)

Immunofluorescence image of rat skin tissue using anti-CD163 (dilution at 1:100)

IHC-P staining of pig lung tissue using anti-CD163 (dilution at 1:200)

Immunohistochemical staining of pig large intestines tissue using anti-CD163 (dilution of primary antibody - 1:200)

WB analysis of human breast cancel 25 (Lane1), breast cancel 24 (Lane2) using CD163 antibody (dilution at 1:100)

IHC-P image of human thyroid carcinoma tissue using CD163 antibody (dilution of primary antibody at 1:500)

IHC-P of human lung adenocarcinoma tissue using CD163 antibody (Dilution of primary antibody 1:200)

Western blot analysis of human cell lysates using CD163 antibody (please check application notes for details)

Immunohistochemical staining of paraffin embedded human colon cancer tissue (orb13303 at 1:200) using CD163 antibody. (Dilution of primary antibody 1:200)

Immunohistochemical staining of human colon cancer tissue using CD163 antibody. (Dilution of primary antibody 1:200)

Immunohistochemical staining of paraffin embedded mouse liver tissue using CD163 antibody. (Dilution of primary antibody 1:200)

IF analysis of mouse skin tissue using CD163 antibody (Dilution of primary antibody 1:100)

Immunofluorescent image of mouse liver tissue using CD163 antibody (Primary antibody at 1:100)

Immunohistochemical staining of mouse liver tissue using anti-CD163 (dilution of primary antibody - 1:200)

Immunofluorescent analysis of mouse lung tissue using CD163 antibody (Primary antibody diluted to 1:100)

Immunohistochemical staining of mouse skin tissue using CD163 antibody (dilution of primary antibody - 1:200)

Western blot analysis of guniea pig lymph (lane 1), rat brain (lane 2), mouse stomach (lane3), rat rectum (lane4), human gallbladder (lane 5), human lung cancer (lane 6) tissue using anti-CD163 (0.5 ug/ml)

IHC-P image of rat lymph tissue using anti-CD163 (2.5 ug/ml)

Immunohistochemical staining of rat lymph tissue using CD163 antibody (2.5 ug/ml)

Immunohistochemical staining of mouse stomach tissue using anti-CD163 (2.5 ug/ml)

IHC-P image of mouse stomach tissue using anti-CD163 (2.5 ug/ml)

Immunohistochemical staining of guinea pig colon tissue using anti-CD163 (2.5 ug/ml)

Immunohistochemical staining of guinea pig colon tissue using anti-CD163 (2.5 ug/ml)

Immunohistochemical staining of paraffin embedded human lung cancer tissue using CD163 antibody (2.5 ug/ml)

Western blot analysis of rat lung tissue using anti-CD163 (dilution of primary antibody at 1:500)