CD16 Antibody

• Catalog Number: orb749494
• Category: Antibodies
• Description: Recognizes a protein of 50-65 kDa, identified as CD16 (Workshop IV; Code N39 ) (also known low affinity Fc receptor III for IgG (FcRIII) or Leu 11). CD16 exists as a polypepetide-anchored from (FCRIIIA or CD16A) on human natural killer (NK) cells and monocytes/ macrophages and as a glycosylphosphatidylinositol (GPI)-anchored form (FcRIIIB or CD16B) on neutrophils. CD16B is polymorphic and the two alleles are termed NA1 and NA2.3 CD16 plays a role in signal transduction, NK cell activation and antibody-dependent cellular cytotoxicity. This mAb has been showed to inhibit the binding of immune complex to NK cells, inhibit cytotoxicity of NK cells, and induce calcium fluxes in NK cells and neutrophils.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: HuNK2
• Tested applications: FACS, Functional Studies, IF
• Reactivity: Human
• Isotype: Mouse IgG2a, kappa
• Immunogen: Human peripheral blood lymphocytes from a NK-leukemia patient were used as the immunogen for the CD16 antibody.
• Dilution range: Functional studies (order BSA/sodium azide-free format),Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This CD16 antibody is available for research use only.
• UniProt ID: P08637
• Storage: Store the CD16 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the CD16 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.