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Anti-Hu CD16 FITC

Catalog Number: orb44655

DispatchUsually dispatched within 5-10 working days.
$ 330.00
Catalog Numberorb44655
CategoryAntibodies
DescriptionMouse Monoclonal to CD16.
TargetCD16
ClonalityMonoclonal
IsotypeMouse IgG1 kappa
ConjugationFITC
ReactivityHuman, Primate
Buffer/PreservativesStabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
PurityPurified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
ImmunogenHuman neutrophils
RRIDAB_10993896
Tested applicationsFC
Application notesFlow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Antibody TypePrimary Antibody
Clone Number3G8
StorageStore at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Alternative namesAnti-CD16 antibody, anti-FACSgammaRIII antibody
NoteFor research use only
Anti-Hu CD16 FITC

Staining pattern of Anti-human CD16 FITC antibody (clone 3G8) in dot-plot fluorescence visualization (lymphocyte gate) Analysis of the antibody staining profile was performed on blood leukocytes isolated from peripheral whole blood by bulk erythrocyte lysis using 10× diluted EXCELLYSE Live. Mouse monoclonal anti-human CD16 FITC antibody (clone 3G8) was used in concentration 9 µg/ml in stained stained blood sample (2 x 10^6 cells) and Mouse monoclonal anti-human CD3 APC antibody (clone UCHT1) in concentration 6 µg/ml, respectively.

Anti-Hu CD16 FITC

Staining pattern of Anti-human CD16 FITC antibody (clone 3G8) in dot-plot fluorescence visualization (mononuclear gate) Analysis of the antibody staining profile was performed on blood leukocytes isolated from peripheral whole blood by bulk erythrocyte lysis using 10× diluted EXCELLYSE Live. Mouse monoclonal anti-human CD16 FITC antibody (clone 3G8) was used in concentration 9 µg/ml in stained stained blood sample (2 x 10^6 cells) and Mouse monoclonal anti-human CD14 PE-Cy™7 antibody (clone MEM-15) in concentration 8 µg/ml, respectively.

Anti-Hu CD16 FITC

Reactivity of Anti-human CD16 FITC antibody (clone 3G8) on human peripheral leukocytes Analysis of the antibody staining profile was performed on blood leukocytes isolated from peripheral whole blood by bulk erythrocyte lysis using 10× diluted EXCELLYSE Live. Suspension of blood leukocytes (2 x 10^6 cells) was added to the mixture of CD16 FITC antibody (clone 3G8, 9 µg/ml in stained blood sample), backbone antibody conjugates and Monocyte Blocking Buffer, vortexed and incubated for 20 min. Stained sample was fixed with 2 ml of 10× diluted EXCELLYSE Easy solution for 10 min. Finally, samples were centrifuged (670 g, 5 min.), supernatant removed and the cell pellet was resuspended in 200 µl of PBS for acquisition.

Anti-Hu CD16 FITC

Anti-human CD16 FITC antibody (clone 3G8) works in flow cytometry application. Analysis of the antibody staining profile was performed on blood leukocytes isolated from peripheral whole blood by bulk erythrocyte lysis using 10× diluted EXCELLYSE Live. Mouse monoclonal anti-human CD16 FITC antibody (clone 3G8) was used in concentration 9 µg/ml in stained blood sample (2 x 10^6 cells).

Anti-Hu CD16 FITC

Flow cytometry multicolor surface staining pattern of human peripheral whole blood stained using anti-human CD16 (3G8) FITC antibody (4 μl reagent / 100 μl of peripheral whole blood) and anti-human CD3 (UCHT1) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Anti-Hu CD16 FITC

Separation of human CD16 positive CD3 negative lymphocytes (red-filled) from CD16 negative CD3 positive lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD16 (3G8) FITC antibody (4 μl reagent / 100 μl of peripheral whole blood).

Anti-Hu CD16 FITC

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD16 (3G8) FITC antibody (4 μl reagent / 100 μl of peripheral whole blood).

  • Anti-Hu CD16 FITC [orb43908]

    FC

    Human, Primate

    Monoclonal

    FITC

    100 T
  • Anti-Hu CD16 FITC [orb44104]

    FC

    Human

    Monoclonal

    FITC

    100 T