Rabbit anti-Thrombomodulin Recombinant Monoclonal Antibody

• Catalog Number: orb1710821
• Category: Antibodies
• Description: Thrombomodulin Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: BLR260L
• Tested applications: FC, ICC, IHC, IP, WB
• Reactivity: Mouse
• Immunogen: Residues 17-517 (ECD)
• Antibody Type: Primary Antibody
• Concentration: 500 µg/ml
• Dilution range: WB: 1:1,000, IP: 12 µl/1 mg lysate, IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections., ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: Thrombomodulin
• UniProt ID: P15306
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: CD141; TM; thrombomodulin; fetomodulin; CD antigen
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of mouse Thrombomodulin by western blot. Samples: Whole cell lysate (50 µg) from mIMCD-3, NIH 3T3, bEnd.3, C2C12, and CH27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Thrombomodulin recombinant monoclonal antibody (orb1710821) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of mouse Thrombomodulin by immunohistochemistry. Sample: FFPE section of mouse gut. Antibody: Rabbit anti-Thrombomodulin recombinant monoclonal antibody (orb1710821). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of mouse Thrombomodulin (shaded) in 7OZ/3 cells by flow cytometry. Antibody: Rabbit anti-Thrombomodulin recombinant monoclonal antibody (orb1710821) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Detection of mouse Thrombomodulin by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from bEnd.3 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-Thrombomodulin recombinant monoclonal antibody (orb1710821) used for IP at 12 µl/mg lysate.

Detection of mouse Thrombomodulin by immunocytochemistry. Sample: FFPE section of mouse 7OZ/3 cells. Antibody: Rabbit anti-Thrombomodulin recombinant monoclonal antibody (orb1710821). Secondary: HRP-conjugated goat anti-rabbit IgG.