CD14 Antibody

• Catalog Number: orb534064
• Category: Antibodies
• Description: Coreceptor for bacterial lipopolysaccharide. In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway. [UniProt]
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: A portion of amino acids 25-148 was used as immunogen for this CD14 antibody.
• UniProt ID: P08571
• Tested applications: IHC-P, WB
• Dilution range: IHC (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 1-2ug/ml
• Application notes: The stated application concentrations are suggested starting points. Titration of the CD14 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Antibody Type: Primary Antibody
• Clone Number: CDLA14-2
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Research Area: Epigenetics, Immunology
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human lymph node with CD14 antibody (clone CDLA14-2). HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.

Western blot testing of human A549 cell lysate with CD14 antibody (clone LPSR/2386). Expected molecular weight: 40-55 kDa depending on glycosylation level.