CD14 Antibody

• Catalog Number: orb606534
• Category: Antibodies
• Description: Recognizes a protein of 55kDa, identified as Cluster of Differentiation 14 (also known lipopolysaccharide receptor or LPSR). CD14 is expressed strongly on monocytes and macrophage and weakly on the surface of neutrophils. It is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for complexes of LPS and LPS binding protein (LBP). Soluble CD14, also binding to LPS, acts at physiological concentration as an LPS agonist and has, at higher concentrations, an LPS antagonizing effect in cell activation.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Purity: Protein G affinity chromatography
• Immunogen: A human recombinant partial protein (within amino acids 25-148) was used as immunogen for this CD14 antibody.
• UniProt ID: P08571
• Tested applications: FACS, IHC-P, WB
• Dilution range: Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Application notes: Variations in protocols, secondaries and substrates may require the CD14 antibody to be titered up or down for optimal performance.
• Antibody Type: Primary Antibody
• Clone Number: LPSR/2386
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: CD14 antibody with azide can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
• Hazard Information: This CD14 antibody is available for research use only.
• Note: For research use only

Flow cytometry testing of human A549 cells with CD14 antibody (clone LPSR/2386); Red = isotype control, Blue = CD14 antibody.

IHC testing of FFPE human tonsil with CD14 antibody (clone LPSR/2386). HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human lymph node tissue with CD14 antibody (clone LPSR/2386). HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.

Western blot testing of human A549 cell lysate with CD14 antibody (clone LPSR/2386). Expected molecular weight: 40-55 kDa depending on glycosylation level.

Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using CD14 antibody (clone LPSR/2386). These results demonstrate the foremost specificity of the LPSR/2386 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free CD14 antibody (clone LPSR/2386) as confirmation of integrity and purity.