CD138/Syndecan-1 (IHC prefer), Extracellular Antibody

• Catalog Number: orb1294300
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Syndecan-1
• Target: CD138 / Syndecan-1 (IHC prefer), Extracellular
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit-IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Synthetic peptide / Extracellular domain corresponding to Human CD138 / Syndecan-1
• Tested applications: IF, IHC, WB
• Dilution range: Western Blot 1:500 Immunofluorescence 1:200 Immunohistochemistry (Paraffin) 1:100
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only

All lanes: Anti-CD138/Syndecan-1 antibody at 1/500 dilution, Lysates/proteins at 60 μg per lane. This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294300 overnight at 4°C.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling CD138/Syndecan-1 with orb1294300 at 1/100.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling CD138/Syndecan-1 with orb1294300 at 1/100.

Cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at RT. Cells were stained with orb1294300 Anti-CD138/Syndecan-1 antibody (red) at 1:200 and 4°C.