CD11a Antibody / LFA-1

SKU: orb749611

Description

Recognizes a protein of 180 kDa, identified as CD11a (Leucocyte Workshop IV; Code 1524). CD11a complex with the beta 2 subunit of the integrin family, CD18, to form the cell surface heterodimer, LFA-1 or CD11a /C18 (aLbL). LFA-1 is expressed on all leukocytes including lymphocytes, monocytes, and granulocytes. It is involved in leukocyte adhesion to its ligands including intercellular adhesion molecule-1 (ICAM-1 or CD54), ICAM-2 (CD102), ICAM-3 (CD50) and Telencephalin (TLN) and play a role in most immune/inflammatory responses. This mAb potently blocks LFA-1 dependent homotypic cell aggregation.

Research Area

Epigenetics, Infectious Diseases

Images & Validation

• Tested Applications: FACS, Functional Studies, IF
• Dilution Range: Flow cytometry: 1-2ug/10^6 cells,Immunofluorescence: 1-2ug/ml,Functional studies (order BSA/sodium azide-free format)
• Reactivity: Human
• Application Notes:
  Optimal dilution of the CD11a antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2b, kappa
• Clone No.: DF1524
• Immunogen: Human CD11a protein was used as the immunogen for the CD11a antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

SDS-PAGE Analysis of Purified, BSA-Free CD11a Antibody (clone DF1524). Confirmation of Integrity and Purity of the Antibody.

Quick Database Links

UniProt

P20701