CD11a Antibody / LFA-1

• Catalog Number: orb248414
• Category: Antibodies
• Description: This antibody recognizes a protein of 180kDa, identified as CD11a. CD11a complexes with the beta 2 subunit of the integrin family, CD18, to form the cell surface heterodimer called LFA-1 or CD11a/CD18. LFA-1 is expressed on all leukocytes including lymphocytes, monocytes, and granulocytes. It is involved in leukocyte adhesion to its ligands including intercellular adhesion molecule-1 (ICAM-1 or CD54), ICAM-2 (CD102), ICAM-3 (CD50) and Telencephalin (TLN) and play a role in most immune/inflammatory responses.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CRIS-3
• Tested applications: FACS, IF
• Reactivity: Human
• Isotype: Mouse IgG2b, kappa
• Immunogen: Stimulated human leukocytes were used as the immunogen for this CD11b antibody.
• Antibody Type: Primary Antibody
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml Functional studies (order BSA/sodium azide-free format)
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This CD11a antibody is available for research use only.
• Entrez: 3683
• Storage: Store the CD11a antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
• Expiration Date: 12 months from date of receipt.

FACS staining of human PBMCs using CD11a antibody (CRIS-3).

SDS-PAGE Analysis of Purified, BSA-Free CD11a Antibody (clone CRIS-3). Confirmation of Integrity and Purity of the Antibody.