Endoglin Antibody / CD105

SKU: orb2640130

Description

CD105/Endoglin is a Type I membrane glycoprotein located on cell surfaces and is part of the TGF-beta receptor complex. This protein has been found on endothelial cells, activated macrophages, fibroblasts, and smooth-muscle cells. Endoglin has a role in the development of the cardiovascular system and in vascular remodeling. Its expression is regulated during heart development. It is highly expressed in endothelial cells during tumor angiogenesis and inflammation, with weak or negative expression in vascular endothelium of normal tissues. Angiogenesis is a promising prognostic marker in a variety of tumors. Endoglin is a more specific and sensitive marker for tumor angiogenesis than CD31 or CD34, as it labels only newly-formed blood vessels and may serve as a prognostic marker for Prostate Adenocarcinoma, and cancers of the lung, stomach, breast, and brain.

Images & Validation

• Tested Applications: WB
• Dilution Range: Western blot: 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Titering of the Endoglin antibody may be required for optimal performance.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: ENG/1327
• Immunogen: A partial recombinant protein corresponding to amino acids 74-251 of human CD105/Endoglin was used as the immunogen for the Endoglin antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Western blot testing of human 1) HeLa and 2) A431 cell lysate with Endoglin antibody (clone ENG/1327). Observed molecular weight: 70/90 kDa (monomer, unmodified/glycosylated); 140-180 kDa (dimer, unmodified/glycosylated).

SDS-PAGE Analysis of Purified, BSA-Free Endoglin Antibody (clone ENG/1327). Confirmation of Integrity and Purity of the Antibody.

Quick Database Links

UniProt

P17813