Endoglin Antibody / CD105

SKU: orb699665

Description

CD105/Endoglin is a Type I membrane glycoprotein located on cell surfaces and is part of the TGF-beta receptor complex. This protein has been found on endothelial cells, activated macrophages, fibroblasts, and smooth-muscle cells. Endoglin has a role in the development of the cardiovascular system and in vascular remodeling. Its expression is regulated during heart development. CD105 is highly expressed in endothelial cells during tumor angiogenesis and inflammation, with weak or negative expression in vascular endothelium of normal tissues. Angiogenesis is a promising prognostic marker in a variety of tumors. Endoglin is a more specific and sensitive marker for tumor angiogenesis than CD31 or CD34, as it labels only newly-formed blood vessels and may serve as a prognostic marker for Prostate Adenocarcinoma, and cancers of the lung, stomach, breast, and brain.

Research Area

Cancer, Epigenetics

Images & Validation

• Tested Applications: ELISA, FACS, IF
• Dilution Range: ELISA: order Ab without BSA for coating,Flow cytometry: 1-2ug/million cells,Immunofluorescence: 2-4ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the Endoglin antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: ENG/1621
• Immunogen: A recombinant extracellular fragment from the human protein was used as the immunogen for the Endoglin antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Flow cytometry testing of PFA-fixed human HeLa cells with Endoglin antibody (clone ENG/1621); Red=isotype control, Blue= Endoglin antibody.

Immunofluorescent staining of PFA-fixed human HeLa cells with Endoglin antibody (clone ENG/1621).

SDS-PAGE analysis of purified, BSA-free Endoglin antibody (clone ENG/1621) as confirmation of integrity and purity.

Quick Database Links

UniProt

P17813