CCND1 Antibody

SKU: orb1925457

Description

Purified Mouse Monoclonal Antibody (Mab)

Research Area

Cancer Biology

Images & Validation

• Tested Applications: WB
• Dilution Range: WB - 1:2000
• Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,κ
• Clone No.: B3061EV779X15X7
• Immunogen: This antibody is generated from a mouse immunized with a recombinant protein from human.
• Target: CCND1 {ECO:0000303|PubMed:8204893, ECO:0000312|HGNC:HGNC:1582}
• Molecular Weight: 33729 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

G1/S-specific cyclin-D1, B-cell lymphoma 1 protein, BCL-1, BCL-1 oncogene, PRAD1 oncogene, CCND1, BCL1, PRAD1

All lanes: Anti-CCND1 at 1:2000 dilution. Lane 1: C6 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Mouse IgG/A/M (H/L), Peroxidase conjugated at 1/2000 dilution.Observed band size: 33kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-CCND1 at dilution. Lane 1: MCF-7 whole cell lysate. Lane 2: HT-1080 whole cell lysate. Lane 3: L929 whole cell lysate. Lane 4: NIH/3T3 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

CCND1 {ECO:0000303|PubMed:8204893, ECO:0000312|HGNC:HGNC:1582}

UniProt

P24385