CCAR2/DBC1 Antibody

SKU: orb1248104

Description

CCAR2/DBC1 Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Porcine, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:64000. Western Blot: Approx 140kDa band observed in nuclear lysates of cell lines HEK293, MCF7 and NIH3T3 (calculated MW of 103kDa according to Human NP_066997.3 and Mouse NP_666167.3). Recommended concentration: 0.5-2ug/ml.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-ESHNRFSATEVTNK
• Target: CCAR2 / DBC1
• Molecular Weight: Approx 140kDa
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CCAR2, cell cycle and apoptosis regulator 2, DBC-1, DBC1, KIAA1967, NET35, p30 DBC, p30DBC, cell cycle and apoptosis regulator protein 2, cell division cycle and apoptosis regulator protein 2, deleted in breast cancer 1, p30 DBC protein

orb1248104 (0.5 ug/ml) staining of HEK293 (A) and MCF7 (B) nuclear lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1248104 (2 ug/ml) staining of NIH3T3 nuclear lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

CCAR2 / DBC1

UniProt

Q8N163

RefSeq (Protein):NP_066997.3