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Catalog Number | orb198491 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to CCAR2 |
Target | CCAR2 / DBC1 (aa842-855) |
Clonality | Polyclonal |
Species/Host | Goat |
Conjugation | Unconjugated |
Reactivity | Bovine, Canine, Human, Mouse, Porcine, Rat |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Protein Sequence | ESHNRFSATEVTNK |
MW | 103 |
Tested applications | ELISA, WB |
Dilution range | ELISA: 1:64000, WB: 0.5-2 μg/ml |
Application notes | This antibody has cross reactivity with Human, Mouse, Rat, Dog, Pig, CowELISA: antibody detection limit dilution 1:64000.WB: Approx 140kDa band observed in nuclear lysates of cell lines HEK293, MCF7 and NIH3T3 (calculated MW of 103kDa according to Human NP_066997.3 and Mouse NP_666167.3). The observed molecular weight is explained by its acetylation (Zheng et al, Mol Cell Biol. 2013 Dec;33(24):4960-70. PMID: 24126058). Recommended concentration: 0.5-2µg/ml. |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti CCAR2 antibody, anti cell cycle and apoptosis Read more... |
Note | For research use only |
Entrez | 57805 |
Western blot analysis of HEK293 (Lane 1) and MCF7 (Lane 2) nuclear lysates using CCAR2 antibody
Western blot analysis of NIH3T3 nuclear lysates using CCAR2 antibody