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CCAR2 Antibody
SKU: orb1256573
Featured
Description
Research Area
Cancer Biology, Cell Biology
Images & Validation
−Item 1 of 9
| Tested Applications | IF, IHC, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 654-923 of human CCAR2 (NP_066997.3). |
| Target | CCAR2 |
| Molecular Weight | Observed: 100kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−CCAR2, cell cycle and apoptosis regulator 2, DBC-1, DBC1, KIAA1967, NET35, p30 DBC, p30DBC, cell cycle and apoptosis regulator protein 2, cell division cycle and apoptosis regulator protein 2, deleted in breast cancer 1, p30 DBC protein
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Western blot analysis of extracts of various cell lines, using CCAR2 antibody (orb1256573) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat brain using CCAR2 antibody (orb1256573) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using CCAR2 antibody (orb1256573) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human rectal cancer using CCAR2 antibody (orb1256573) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using CCAR2 antibody (orb1256573) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using CCAR2 antibody (orb1256573) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using CCAR2 antibody (orb1256573) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse stomach using CCAR2 antibody (orb1256573) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using CCAR2 antibody (orb1256573). Blue: DAPI for nuclear staining.
Quick Database Links
Gene Symbol
CCAR2
UniProt
UniProt Details
− No UniProt data available
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
CCAR2 Antibody (orb1256573)
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