CAT Antibody (Center)

• Catalog Number: orb1928389
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Target: This CAT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 152-180 amino acids from the Central region of human CAT.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Porcine, Rat, Zebrafish
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: P04040
• MW: 59756 Da
• Tested applications: FC, IHC-P, WB
• Dilution range: WB: 1:2000, IHC-P: 1:50~100, FC: 1:10~50
• Antibody Type: Primary Antibody
• Clone Number: RB21613
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Catalase, CAT
• Note: For research use only
• NCBI: NP_001743.1

CAT Antibody (Center) flow cytometric analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human lung carcinoma reacted with CAT Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

All lanes: Anti-CAT Antibody (Center) at 1:2000 dilution. Lane 1: HepG2 whole cell lysate. Lane 2: Mouse kidney lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 60 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.