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Catalog Number | orb1537896 |
---|---|
Category | Antibodies |
Description | CASZ1 Antibody |
Target | CASZ1 |
Clonality | Polyclonal |
Species/Host | Rabbit |
Isotype | IgG |
Conjugation | Unconjugated |
Reactivity | Bovine, Human, Mouse, Primate, Rat |
Concentration | 1 mg/ml |
Buffer/Preservatives | 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide. 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide |
Purification | Affinity purified |
Immunogen | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to region near the amino terminal end of human Cas5 protein. |
Tested applications | ELISA, IHC, IHC-P, WB |
Dilution range | ELISA (1:10000 - 1:50000), IHC, IHC-P (5 µg/ml), WB (1:500 - 1:2000) |
Application notes | Further information: Immunohistochemistry: LS-B2838 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2838 was determined to be 5 ug/ml. Western Blot: A band approximately 125 kD in size corresponding to hCas5 protein is expected in the appropriate cell lysate or extract. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | CASZ1, Castor-related protein, CAS11, Castor, Cast Read more... |
Note | For research use only |
IF, IHC-Fr, IHC-P | |
Human | |
Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
WB | |
Bovine, Equine, Guinea pig, Human, Rat | |
Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
WB | |
Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Zebrafish | |
Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |