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Caspase-3 Antibody, Pro and Active

Catalog Number: orb248113

DispatchUsually dispatched within 5-10 working days
$ 370.00
Catalog Numberorb248113
CategoryAntibodies
DescriptionCaspases, or cysteine-aspartic proteases, are a family of cysteine proteases that play essential roles in inflammation, apoptosis and necrosis. They are often referred to as the 'executioners of apoptosis.' Caspases are synthesized as inactive proenzymes comprising an N-terminal peptide (prodomain) together with one large and one small subunit. Active caspase-1 and -3 are heterodimers, containing two small and two large subunits. Active Caspase-3 subunits are 17kD (p17) and 12kD (p12). Pro Caspase-3 is inactive and has virtually no activity until it is cleaved by an initiator caspase. It is activated by Caspases -8, -9 and -10 ('initiator caspases') and then effects apoptosis by cleaving targeted cellular proteins.
Species/HostMouse
ClonalityMonoclonal
Clone NumberCAS3836
Tested applicationsIHC-P, WB
ReactivityHuman, Mouse, Rat
IsotypeMouse IgG1, kappa
ImmunogenThe large subunit domain of human Caspase-3 was used as the immunogen for this monoclonal antibody.
Antibody TypePrimary Antibody
Dilution rangeWestern blot: 2-4ug/ml,IHC (FFPE): 5ug/ml
PurityProtein G purified monoclonal antibody
ConjugationUnconjugated
Formula0.5 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Hazard InformationThis Caspase-3 antibody is available for research use only.
Entrez836
StorageAliquot the Pro and Active Caspase-3 antibody and store frozen at -20°C or colder to avoid repeated freeze-thaw cycles.
Buffer/Preservatives1X PBS, pH 7.4
NoteFor research use only
Application notesThis antibody will detect the pro form and p17 and p12 active forms of Caspase-3.
Expiration Date12 months from date of receipt.
Caspase-3 Antibody, Pro and Active

Western blot testing of staurosporine-treated Jurkat cells (2 hr, 2 uM) with Caspase-3 antibody at 2 ug/ml. The pro form is seen at ~32kD and active caspase-3 seen at ~17 kDa and ~12 kDa.

Caspase-3 Antibody, Pro and Active

IHC testing of FFPE human colon tissue with Caspase-3 antibody at 5 ug/ml. HIER: steam tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

  • Caspase-3 Antibody, Pro and Active [orb2637448]

    IHC-P,  WB

    Human, Mouse, Rat

    Mouse

    Monoclonal

    Unconjugated

    100 μg
  • Caspase-3 (Pro and Active) Antibody [CAS3836] [orb1252886]

    WB

    Human, Mouse, Rat

    Mouse

    Monoclonal

    Unconjugated

    100 μg