Caspase-3 Antibody, Pro and Active

SKU: orb248113

Description

Caspases, or cysteine-aspartic proteases, are a family of cysteine proteases that play essential roles in inflammation, apoptosis and necrosis. They are often referred to as the 'executioners of apoptosis.' Caspases are synthesized as inactive proenzymes comprising an N-terminal peptide (prodomain) together with one large and one small subunit. Active caspase-1 and -3 are heterodimers, containing two small and two large subunits. Active Caspase-3 subunits are 17kD (p17) and 12kD (p12). Pro Caspase-3 is inactive and has virtually no activity until it is cleaved by an initiator caspase. It is activated by Caspases -8, -9 and -10 ('initiator caspases') and then effects apoptosis by cleaving targeted cellular proteins.

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution range: Western blot: 2-4ug/ml,Immunohistochemistry (FFPE): 5ug/ml
• Reactivity: Human, Mouse, Rat
• Application Notes:
  This antibody will detect the pro form and p17 and p12 active forms of Caspase-3.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: CAS3836
• Immunogen: The large subunit domain of human Caspase-3 was used as the immunogen for this monoclonal antibody.
• Purification: Protein G purified monoclonal antibody
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 1X PBS, pH 7.4
• Disclaimer: For research use only

Western blot testing of staurosporine-treated Jurkat cells (2 hr, 2 uM) with Caspase-3 antibody at 2 ug/ml. The pro form is seen at ~32kD and active caspase-3 seen at ~17 kDa and ~12 kDa.

IHC testing of FFPE human colon tissue with Caspase-3 antibody at 5 ug/ml. HIER: steam tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.