CASP8 Antibody

• Catalog Number: orb625439
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Caspase 8
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: caspase 8, apoptosis-related cysteine peptidase
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:200-1:2000; IP: 1:200-1:2000; IHC: 1:50-1:100; IF: 1:10-1:100
• Form/Appearance: liquid
• Purity: ≥95% as determined by SDS-PAGE
• Conjugation: Unconjugated
• MW: 50-55 kDa, 25-32 kDa
• Target: CASP8
• UniProt ID: Q14790
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Apoptotic cysteine protease, Apoptotic protease Mc
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using orb625439 (Caspase 8 Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

IP Result of anti-CASP8 (IP: orb625439, 3 ug; Detection: orb625439 1:500) with HeLa cells lysate 2500 ug.

HepG2 cells were subjected to SDS PAGE followed by western blot with orb625439 (CASP8 antibody) at dilution of 1:1000.

Immunohistochemistry of paraffin-embedded human lymphoma using orb625439 (CASP8 antibody) at dilution of 1:50.