CASP3 Rabbit Polyclonal Antibody

SKU: orb625436

Description

Rabbit polyclonal antibody to Caspase 3

Research Area

Apoptotic, Cancer, Epigenetics, Neuroscience, Signaling Pathways

Images & Validation

• Tested Applications: ELISA, IF, IHC, IP, WB
• Dilution Range: WB: 1:200-1:2000; IP: 1:200-1:2000; IHC: 1:50-1:500; IF: 1:20-1:200
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: caspase 3, apoptosis-related cysteine peptidase
• Target: CASP3
• Molecular Weight: 32-35 kDa
• Purity: ≥95% as determined by SDS-PAGE
• Purification: Immunogen affinity purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: liquid
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Apopain, CASP 3, CASP3, Caspase 3, Caspase 3/p17, Caspase3, CPP 32, CPP32, CPP32B, Cysteine protease CPP32, Protein Yama, SCA 1, SREBP cleavage activity 1

Immunofluorescent analysis of (-20℃ Ethanol) fixed NIH/3T3 cells using orb625436 (Caspase 3 Antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).

IP result of anti-Caspase 3 (IP: orb625436, 4 ug; Detection: orb625436 1:300) with NIH/3T3 cells lysate 3440 ug.

HeLa cells were subjected to SDS PAGE followed by western blot with orb625436 (CASP3 antibody) at dilution of 1:1000.

Immunohistochemistry of paraffin-embedded human spleen using orb625436 (CASP3 antibody) at dilution of 1:50.

Quick Database Links

Gene Symbol

CASP3

UniProt

P42574

Tayebeh Mohtashami et al. Neuroprotective Effects of Platelet-Derived Exosomes in a Rat Model of Methamphetamine-Induced Neurotoxicity Mol Neurobiol ., (2025)

PubMed: 41247551

Applications

IHC

Reactivity

Rat