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Catalog Number | orb1248560 |
---|---|
Category | Antibodies |
Description | cardiac troponin T Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Predicted Reactivity | Bovine, Canine, Feline |
Reactivity | Human, Mouse, Porcine, Rat |
Immunogen | The immunogen for this antibody is: C-RNRINDNQKVSKT |
Concentration | 500 ug/mL |
Dilution range | Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 40kDa band observed in Human, fetal Mouse and adult Rat and Pig Heart lysates (calculated MW of 35.6kDa according to Human NP_000355.2 and 35.7kDa according to Rat NP_036808.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Luedde et al, Cardiovasc Res. 2010 Jun 1;86(3):452-60. PMID:20083571). Recommended concentration: 0.03-0.1ug/ml. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Approx 40kDa |
Target | cardiac troponin T |
UniProt ID | P45379 |
NCBI | NP_001001430.1, NP_001001432.1, NP_000355.2, NP_001001431.1 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | TNNT2, troponin T type 2 (cardiac), CMH2, CMPD2, L Read more... |
Note | For research use only |
Application notes | Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 40kDa band observed in Human, fetal Mouse and adult Rat and Pig Heart lysates (calculated MW of 35.6kDa according to Human NP_000355.2 and 35.7kDa according to Rat NP_036808.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Luedde et al, Cardiovasc Res. 2010 Jun 1;86(3):452-60. PMID:20083571). Recommended concentration: 0.03-0.1ug/ml. |
Expiration Date | 12 months from date of receipt. |
orb1248560 (0.03 ug/ml) staining of Rat Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
orb1248560 (0.01 ug/ml) staining of Pig Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
ELISA, IF, IHC, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IH, WB | |
Human, Mouse, Rabbit, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, WB | |
Bovine, Canine, Feline, Human, Mouse, Porcine, Rat | |
Goat | |
Polyclonal | |
Unconjugated |
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