cardiac troponin T Antibody

• Catalog Number: orb1248072
• Category: Antibodies
• Description: cardiac troponin T Antibody
• Target: cardiac troponin T
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Porcine
• Predicted Reactivity: Bovine, Canine, Feline, Rat
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-TERKSGKRQTERE
• UniProt ID: P45379
• MW: Approx 40kDa
• Tested applications: ELISA, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 40kDa band observed in Human, Mouse and Pig Heart lysates (calculated MW of 35.6kDa according to Human NP_000355.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Luedde, Cardiovasc Res. 2010 Jun 1;86(3):452-60. PMID: 20083571). Recommended concentration: 0.1-0.3ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: TNNT2, troponin T type 2 (cardiac), CMH2, CMPD2, L
• Note: For research use only
• NCBI: NP_001001431.1, NP_001001432.1, NP_001001430.1, NP_000355.2

orb1248072 (0.1 ug/ml) staining of Human (A) and Mouse (B) Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1248072 (0.1 ug/ml) staining of Pig Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.