carbonic anhydrase XII Antibody

• Catalog Number: orb1248281
• Category: Antibodies
• Description: carbonic anhydrase XII Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-SHLQHVKYKGQE
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 45kDa band observed in Human Kidney lysates (calculated MW of 39.5kDa according to NP_001209.1). Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:In paraffin embedded Human Kidney shows membranous staining in some epithelial cells of convoluted tubules. Recommended concentration, 5-10ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Approx 45kDa
• Target: carbonic anhydrase XII
• UniProt ID: O43570
• NCBI: NP_996808.1, NP_001209.1, NP_001280571.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: CA12, carbonic anhydrase XII, CAXII, HsT18816, CA-
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 45kDa band observed in Human Kidney lysates (calculated MW of 39.5kDa according to NP_001209.1). Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:In paraffin embedded Human Kidney shows membranous staining in some epithelial cells of convoluted tubules. Recommended concentration, 5-10ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1248281 (0.3 ug/ml) staining of Human Kidney lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1248281 (5 ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.