Goat anti-carbonic anhydrase XII (aa188-199) Antibody

• Catalog Number: orb167716
• Category: Antibodies
• Description: Goat polyclonal antibody to CA12.
• Target: carbonic anhydrase XII (aa188-199)
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: SHLQHVKYKGQE
• MW: 39.5; 38.4; 31.9
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:128000, WB: 0.3-1 μg/ml, IHC-P: 5-10µg/ml
• Application notes: WB: Approx 45kDa band observed in Human Kidney lysates (calculated MW of 39.5kDa according to NP_001209.1). Recommended concentration: 0.3-1µg/ml.. IHC: In paraffin embedded Human Kidney shows membranous staining in some epithelial cells of convoluted tubules. Recommended concentration, 5-10µg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti CA12 antibody, anti carbonic anhydrase XII an
• Note: For research use only
• Entrez: 771

Western blot analysis of Human Kidney lysate (35ug protein in RIPA buffer) using CA12 antibody

Immunohistochemical staining of Human Kidney using CA12 antibody