CAPZB Antibody

• Catalog Number: orb1247707
• Category: Antibodies
• Description: CAPZB Antibody
• Target: CAPZB
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Porcine, Rat
• Predicted Reactivity: Bovine, Canine
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: NDLVEALKRKQQC
• UniProt ID: P47756
• Tested applications: ELISA, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 30kDa band observed in lysates of cell lines Jurkat, HeLa and NIH-3T3 and approx 28kDa band observed in Mouse, Rat and Pig Heart lysates (calculated MW of 30.6kDa according to Human NP_004921.1 and 31.3kDa according to Mouse NP_001032850.1, and 30.6kDa according to Rat NP_001005903.1 and Pig NP_001106915.1]. Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour. This product has been successfully used in WB on Rat: Wang CY et al, J Biomed Sci. 2011 Feb 7;18:13. PMID: 21299884.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CAPZB, capping protein (actin filament) muscle Z-l
• Research Area: Cancer Biology
• Note: For research use only
• NCBI: NP_004921.1

orb1247707 (0.1 ug/ml) staining of Mouse (A), Rat (B) and Pig (C) Heart lysates (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247707 (0.01 ug/ml) staining of Jurkat (A), HeLa (B) and NIH-3T3 (C) lysates (35 ug protein in RIPA buffer). Detected by chemiluminescence